Unless otherwise advertised, all HSR routes are scheduled to operate service 7 days per week throughout the year with the exception of the following:
- 9 ROCK GARDENS (Sunday & Holiday Only) - Service in effect from Sunday, May 9, 2010 to Sunday, November 7, 2010;
- 10 B-LINE (Weekday Only);
- 12 WENTWORTH (Weekday Only);
- 16 ANCASTER (Weekday & Saturday Only);
- 18 WATERDOWN (Weekday Only);
- 20 A-LINE (Weekday Only);
- 43 STONE CHURCH (Weekday & Saturday Only);
- 44 RYMAL (Weekday Only);
- 51 UNIVERSITY (Weekday & Saturday Only) - No service between May and August and during McMaster reading week in February;
- 52A DUNDAS LOCAL (Weekday Only);
- 56 CONFEDERATION PARK (Saturday, Sunday & Holiday Only - weather dependent) - Service in effect from Saturday, June 5, 2010 to Monday, September 6, 2010.
- 99 WATERFRONT SHUTTLE (Weekday, Saturday & Sunday) - Summer service only.